摘要
目的 :寻找与海洛因依赖有关的特异性基因。方法 :用递增法建立海洛因依赖小鼠模型。纳洛酮催瘾后 ,分别提取实验组及对照组小鼠脑内的总RNA、mRNA ,然后进行抑制性消减杂交 ,杂交产物扩增 ,凝胶电泳分析。结果 :实验组小鼠脑内mRNA含量高于对照组 ,并于实验组小鼠脑内分离出差异表达的cDNA片段。结论 :海洛因依赖小鼠脑内可能存在特异性基因表达。
Objective:To find out the specific gene related to heroin dependence.Methods:Heroin-dependent models in mice were established by dose-increasing administration.After withdrawal syndrome was precipitated by naloxone,total RNA and mRNA were isolated from the brains of experimental and control groups respectively,then suppressive subtractive hybridization was proceeded.The hybridization sample was amplified by PCR and analyzed on a agarose/EB gel.Results:The mRNA level of the experimental group was significantly higher than that of control group and a differently expressing cDNA fragment was obtained from the brain of the experimental group.Conclusion:There is probably an expression of the specific gene in the brain of heroin-dependent mice.
出处
《重庆医科大学学报》
CAS
CSCD
2003年第5期625-627,共3页
Journal of Chongqing Medical University
基金
重庆市科委应用基础基金资助项目 ( 99-16-110 )
关键词
海洛因依赖
MRNA
抑制性消减杂交
Heroin dependence
mRNA
Suppressive subtractive hybridization
