摘要
目的:观察大鼠一侧大脑中动脉闭塞(MCAO)后远隔部位(两侧额叶、丘脑、小脑细胞形态学变化)以探讨其发病机制。方法:健康Sprague-Dawley大鼠20只,体质量250～300g,采用线栓法建立大鼠一侧大脑中动脉闭塞模型,按实验需要将实验动物分成正常、单纯缺血两组;用苏木精-伊红染色和末端脱氧核苷酸转移酶介导的d-UTP生物素标记(TUNEL)法观察两侧额叶、丘脑、小脑MCAO后6,24h、1周细胞形态学变化。结果:同侧额叶、丘脑MCAO后6h开始出现TUNEL阳性细胞分别为(34.3±4.0),(42.4±3.0)个,24h后明显增加犤分别为(198.0±2.6),(239.8±4.2)个犦,至1周阳性细胞减少犤分别为(20.0±1.2),(22.2±2.6)个(F=5272.9,5749.4,P<0.05)犦,而对侧丘脑、额叶及两侧小脑未见明显细胞形态学改变。结论:一侧脑梗死后,运动功能的恢复可由脑卒中后远隔部位脑组织功能所促进,提示神经功能联系不能的恢复在脑卒中的康复中起关键作用。
AIM:To observe the cell morphology of bilateral frontal lobes, thalami and cer ebellums in rats after unilateral middle cerebral artery occlusion (MCAO) and to investigate its pathogenesis. METHODS:Twenty Sprague-Dawley rats weighing 250-300 g were divided into cont rol group (n=5) and ischemia group (n=15).Unilateral MCAO model was established with thread occlusion. Hematoxylin and eosin (HE) staining and terminal dUTP nic k and labeling (TUNEL) were used to observe the cell morphology at 6, 24 h, 1 d and 1 week after MCAO. RESULTS:TUNEL positive cells in frontal lobe and thalamus of the same hemisphe re were found at 6 h after MCAO(34.3±4.0 and 42.4±3.0 respectively), increas ed significantly at 24 h(198.0±2.6 and 239.8±4.2 respectively), and then dec reased at 1 week(20.0±1.2 and 22.2±2.6 respectively)(F=5 272.9,5 749.4,P< 0.05).There were insignificant changes of cell morphology in contralateral tha lamus, lobe and bilateral cerebellums.
出处
《中国临床康复》
CSCD
2003年第25期3414-3415,T001,共3页
Chinese Journal of Clinical Rehabilitation
关键词
局灶性脑缺血
病理改变
发病机制
细胞形态学
Brain tissue function in remote region improves the recovery of mo tor function after unilateral cerebral infarction. It is indicated that the reco very of diaschisis play a key role in the rehabilitation of stroke.
