摘要
用紫外光谱法测定了β-谷甾醇和豆甾醇与Deniges磺基醋酸汞络合物的紫外光谱,探讨了显色反应时间、显色剂用量及浓硫酸与冰醋酸配比对紫外吸收光谱的影响。结果表明在一定浓度范围内,甾醇络合物的吸光度A与其浓度成线性关系。并利用紫外光谱法测定了混合甾醇中β-谷甾醇和豆甾醇的含量,此法所得结果与用气相色谱法测试的结果相近。
The UV spectra of the complex compound of β-sitosterol or stigmasterol with Deniges reagent were measured. The effects of the time for color development reaction, the quantity of colored indicator, and the volume ratio of H2SO4 to HAc on the UV spectra were studied. The experimental results show that the UV spectra of these two complex compounds are different, and there exists a linear relationship between the absorbance A and β-sitosterol or stigmasterol content in a certain concentration range. There is a good agreement between the results obtained by UV spectral and GC methods.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2003年第5期933-936,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(20176047)
江苏省教育厅自然科学基金项目(99KJB150007)
江苏省(青蓝工程"跨世纪学术带头人基金资助
