摘要
在pH 5.0的NaAc-HAc缓冲液中壳聚糖对茜素红S的荧光强度具有明显的猝灭作用,且猝灭程度F0/F与加入的壳聚糖浓度成线性关系,据此建立一种测定壳聚糖含量的荧光猝灭法。确定了最佳测定条件,线性回归方程为F0/F=-0.438 37+0.436 27c(mg·L-1),线性范围为0-16.667 mg·L-1,r=0.996,检测限为0.473 mg·L-1;平均回收率为100.60％,测定了样品中壳聚糖的含量,并考察了干扰因素对测定的影响。采用该方法可测定复杂样品中微量壳聚糖含量。
A new method for the determination of chitosan (CTS) with alizarin red S (ARS) is described. The method is based on the fluorescence quenching effect of CTS and ARS in the buffer of NaAc-HAc at pH 5.0. The best determination condition has been worked out. The linear equation of the fluorescence quenching is: F-0/F= -0.438 37+0.436 27c(mg.L-1) (r=0.996). The linear range is 0-16.667 mg.L-1, the detection limit is 0.473 mg-L-1, and the mean recovery is 100.60%. Influence of a series of reagents on the fluorescence quenching effect of CTS and ARS was also studied. The quantities of CTS in the different compound samples were determined in this way.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2003年第5期895-898,共4页
Spectroscopy and Spectral Analysis
