摘要
本文利用荧光猝灭法,生理pH值7.43±0.02,28℃时,以Cu(Ⅱ)-牛血清白蛋白(BSA)和Zn(Ⅱ)-BSA单金属体系作参考,研究了Cu(Ⅱ)-BSA-Zn(Ⅱ)双金属体系中Cu(Ⅱ),Zn(Ⅱ)与BSA相互作用。在Stern-Volmer猝灭公式的基础上近似推导了此双金属体系中荧光猝灭的缔合公式。与单金属体系的对比后,推测Zn(Ⅱ)会竞争Cu(Ⅱ)与BSA结合,Cu(Ⅱ)与BSA结合的稳定性大于Zn(Ⅱ)与BSA结合的稳定性。
Fluorescence quenching of bovine serum albumin (BSA) by its interaction with Cu(Ⅱ) and Zn(Ⅱ) or with Cu(Ⅱ) alone was observed at 28℃ and physiologically pH 7.43(±0.02) applying fluorescence spectra method. The Stern-Volmer's K'sv cu.BSA, and Lineweaver-Burk's K'D CU-BSA were calculated in these two kinds of systems. The different interactions of Cu(Ⅱ) and Zn(Ⅱ) with BSA were discussed in this paper. By contrasting the single metal system with the double-metal system, the binding of Cu(Ⅱ) and BSA must be strengthened by the competition of Zn(Ⅱ).
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2003年第5期892-894,共3页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金(批准号:29961001)
广西十百千人才基金以及广西高校科学基金资助
