摘要
分别以水、体积分数为50%的乙醇、加酶水和加酶的体积分数为50%的乙醇为溶剂,采用超声波提取大蒜,用Folin-Ciocalteu法测定提取物中总酚含量,研究提取物对DPPH.、.OH和O2的清除能力.实验结果表明:提取剂水、体积分数为50%的乙醇、加酶水和加酶的体积分数为50%的乙醇的超声波在100 g大蒜的提取物中总酚含量依次为112.7、102.4、171.1和130.8 mg.4种提取物对DPPH.、.OH和O2均具有较强的清除能力,表明其均具有较强的抗氧化活性.大蒜提取物抗氧化活性与其总酚含量呈明显的量效关系.
In this paper, ultrasonic-assisted extraction was used to extract activated components from garlic by water, 50% ethanol, water including cellulase and 50% ethanol including cellulase, respectively. The total contents of phenol from the extracts was determined using Folin-Ciocalteu method and the clearing ability of the extracts on DPPH.,.OH and O 2 was studied. The results indicated that under ultrasonic radiation, using water, 50% ethanol, water including cellulase or 50% ethanol including cellulase as extractants, the total contents of phenol was 112.7 mg, 102.4 mg, 171.1 mg and 130.8 mg per 100 g garlic, respectively. All four extracts had strong clearing ability on DPPH-, -OH and O2, which showed they had strong anti-oxidant capacity. In conclusion, the anti-oxidation ability is proportional to the total phenol contents of the garlic extracts.
出处
《江西师范大学学报（自然科学版）》
CAS
北大核心
2011年第6期570-573,共4页
Journal of Jiangxi Normal University(Natural Science Edition)
基金
山西省高校科技开发研究(200611041)资助项目
关键词
大蒜
超声波
纤维素酶
总酚
抗氧化
garlic
ultrasonic wave
cellulase
total phenol
antio-xidation
