摘要
目的:评价树突状细胞负载凋亡肝癌细胞后的免疫应答. 方法:用放线菌素-D诱导肝癌细胞调亡,在体外用负载凋亡肝癌细胞的DC诱导自身淋巴细胞,使其活化为肝癌特异性T细胞,并用其进行杀伤实验.结果:DC负载凋亡肝癌细胞后活化的CTL细胞分泌较高水平Th1型细胞因子IL-12和TNF,并表现出较高的杀伤活性,对NK细胞敏感的靶细胞慢性髓原性白血病细胞K562也有较强的杀伤作用,提示此CTL同时具备NK特征.结论:负载凋亡肝癌细胞的DC对原发性肝癌的研究和治疗具有潜在应用价值.
AIM: To estimate the immune responses of dendritic cells after loaded with apoptotic hepatocellular carcinoma cells. METHODS: IApoptosis of hepatocellular carcinoma cells (HCCs) was induced with actinomycin-D. Interactions of DCs and apoptotic HCCs were observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. RESULTS: After stimulated by apoptotic HCCs pulsed DCs, the culture medium of primed lymphocytes contained a a high level of Th1 cytokines IL-12 and TNF. Lymphocytes primed by apoptotic HCCs pulsed DC were not only specific lysis HCC lines but also K562 cells, and showed a charac- teristic of NK cells. DCs inhibited the growth of human HCC lines and other cancer cells. CONCLUSION: Apoptotic HCCs pulsed DCs have a potential prospect in research and treatment in primary hepato- cellular carcinoma.
出处
《世界华人消化杂志》
CAS
2003年第9期1365-1368,共4页
World Chinese Journal of Digestology
