摘要
目的:观察MUC1基因免疫对H22肝癌生长的特异性抑制作用。方法:采用股四头肌肌肉注射法将构建的MUCl基因疫苗pcDNA3.1-MUC1免疫 Balb/c小鼠,每次 100μg,3 wk/次,共3次,最后一次基因免疫后 3wk,接种表达MUC1的H22肝癌细胞。2 wk后观察、记录肿瘤的生长情况。于肿瘤细胞接种后43 d,处死全部动物,称肿瘤的质量。荷瘤小鼠的瘤组织常规HE染色。结果:肿瘤细胞接种后43d,MUC1预防组,质粒pcDNA3.1对照组及生理盐水阴性对照组H22肝癌大小分别为547±59mm^3,1185±84mm^3和1220±95 mm^3(P<0.01);平均瘤质量分别为 1.87 ± 0.96 g,4.19 ±1.34 g和4.23±1.32 g(P<0.01);pcDNA3.1对照组和生理盐水阴性对照组100％可见瘤体形成,肿瘤生长,而MUC1基因疫苗预防组仅见50％(5/10)的小鼠有瘤体形成,与对照组相比,MUC1预防组H22肝癌生长受到明显抑制(P<0.01);MUC1预防组小鼠免疫保护有显著差异(P<0.05).病理学检查结果显示,与pcDNA3.1对照组相比,MUC1 DNA疫苗预防组鼠H22肝癌组织大量坏死。结论:MUC1基因免疫显著抑制H22肝癌生长。
AIM: To investigate the special anti-H22 hepatocellular carcinoma growth effect of the MUC1 gene immunization. METHODS: Balb/c mice were immunized intramuscularly with 100 μg MUC1 cDNA 3 times at 3-weekly intervals. Three weeks after the last immunization, tumor challenge experiments were performed by using MUC1 expressing tumor cell line H22. Tumor growth inhibition and body protection were observed two weeks later. After 43d of challenge experiments, all mice were killed and tumors were weighed. histological analysis of tumor tissue was carried out with HE staining. RESULTS: After 43 d of challenge experiments, the volumes of H22 hepatocellular carcinoma in MUC1cDNA, pcDNA3.1 (+) and NS groups were 547±59mm^3, 1185±84 mm^3 and 1220±95 mm^3 (P<0.01), respectively. The average mass of H22 hepatocellular carcinoma in the three groups was 1.87±0.96 g, 4.19±1.34 g and 4.23±1.32 g (P<0.01), respectively. Tumorigenic rate was only 50 % in MUC1cDNA group, and was 100 % in pcDNA3.1(+) and NS group. H22 hepatocellular carcinoma growth in mice of MUC1cDNA group was significantly suppressed (P<0.01), and a significant body protective effect was found in mice of MUC1cDN4 group (P<0.05), compared with control group. Histological analysis showed that the H22 hepatocellular carcinoma tissues were markedly necrosed in mice of MUC1cDNA group compared with that in control group. CONCLUSION: MUC1 gene immunization can significantly suppress H22 hepatocellular carcinoma growth.
出处
《世界华人消化杂志》
CAS
2003年第9期1322-1325,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金
No.39470683
