摘要
以前的研究报导了将人肿瘤坏死因子基因(TNF-α)成功转入鱼腥藻7120中,这项研究在实验室小规模范围内探讨了其转TNF-α基因鱼腥藻7120的适宜生长条件。结果表明,转基因鱼腥藻7120最适生长温度在25～30℃,最适pH7.0～7.5。不同光照强度下的净光合放氧速率测定结果表明,转基因鱼腥藻7120与野生型具有一致的光饱和点和光补偿点,且适合在10～700μE.m-2.s-1下生长。外加有机碳源(4g/L葡萄糖)一定程度上可以增加转基因鱼腥藻7120生长速度。对不同藻龄转基因鱼腥藻7120中TNF-α的累积量的检测发现:生长8d左右时转基因鱼腥藻7120中TNF-α累积量达到最大值。这将为产业化生产、适时收获转基因蓝藻提供理论指导,同时讨论了葡萄糖对转基因鱼腥藻7120代谢的调节。
Our previous work presented the construction of transgenic microalgae Anabaena sp. PCC7120 (A. 7120) with human tumor necrosis factorα (hTNFα) gene. We currently described the preliminary studies on optimal growth conditions for transgenic A. 7120 in the laboratoryscale. The data we provided here showed that the optimal growth temperature and pH are at 2530℃, and pH 7.07.5, respectively; the net photosynthetic oxygen elution rates of the transgenic A. 7120 under different photon flux densities measured here suggested that both the saturated and compensated light densities of the transgenic A. 7120 are similar to those of the wild type of A. 7120, and the transgenic and wild type A. 7120 are able to grow normally under 10700 μE·m-2·s-1 with a range of different cell densities. But addition of glucose (4 g/L) into BG11 culture medium increases the growth rates of the transgenic A. 7120 in contrast to the wild type. The contents of TNFα in the different culture ages of the transgenic A. 7120 determined in this investigation suggested that the accumulations of TNFα in the transgenic A. 7120 cultures reached the maxima at about the eighth day. This provides a helpful message for production of TNFα. We proposed here that glucose might play a possible role in controlling the metabolism of
出处
《武汉植物学研究》
CSCD
2003年第5期406-410,共5页
Journal of Wuhan Botanical Research
基金
得到国家海洋863项目资助(819-04-11)
中国科学院武汉植物研究所所长基金支持。
