摘要
小麦种子从萌动至两叶期,用牛胸腺DNA分次进行滴浸处理。一代(D_1)株未出现明显变异,二代(D_2)株在蛋白含量、抗病性、株型、穗型、粒型和成熟期等方面均出现较大变异。在不同的年份间,用相同的缓冲液、技术,将牛DNA处理同一品种时,分年份处理的二代株中均出现性状相似的矮杆大穗变异类型,表现了该类型变异的重演性,其频率为1.95%～2.5%。经对D_1、D_2代变异株根尖和D_1代芽鞘细胞学观察,有染色体畸变存在。这表明牛胸腺DNA引入受体细胞后,可能在多方面作用于受体基因组,表现出复杂的综合效应。
From germination to two-leaf stage, wheat seeds were trickled several times with liquor of thymus DNAs of calf. The treatment caused no obvious variation for D_1 plants. But for D_2 plants, a great number of variations appeared in protein content, disease resistance, plant type, ear type, grain type, maturation and so on. Among the D_2 plants produced in different years after treatment with the identical buffer, DNA and receptor and by the same procedure occurred the same drawf-stem-and-big-ear variation type, proving this type being repeatable, with variation frequency of 1.95～2.5%. Presence of chromosomal aberration, as revealed by cytological observation of root tips and coleoptiles from D_1 and D_2 variants, indicated that the introduction of calf thymus DNA into receptor cells might act on the receptor genomes in various aspects, showing complicated and comprehensive effects.
出处
《山东农业大学学报（自然科学版）》
CSCD
1992年第3期213-220,共8页
Journal of Shandong Agricultural University：Natural Science Edition
基金
山东省教委资助
关键词
牛胸腺
DNA
小麦
定向变异
thymus DNA of calf
wheat
directive variation
