摘要
目的 探讨电磁脉冲 (EMP)辐射对小鼠生精细胞能量代谢的影响。方法 6～ 8周龄二级雄性昆明小鼠 114只 ,随机分为辐射组和对照组。辐射组小鼠分别接受 8× 10 3 V/m ,2× 10 4V/m和 6× 10 4V/mEMP全身重复照射 5次 ,于照射后 6h ,1d ,3d ,7d ,14d和 2 8d ,检测睾丸组织乳酸脱氢酶 (LDH)、琥珀酸脱氢酶 (SDH)、谷氨酸脱氢酶 (GDH)和山梨醇脱氢酶 (SODH)活性 ,外周血红细胞及其参数、血红蛋白浓度 ,同时观察睾丸组织的病理变化。结果 3种场强的EMP照射后 2 8d内 ,各级生精细胞水肿、坏死和脱落 ,精子减少或缺失 ,间质水肿 ,蛋白水肿液积聚 ,毛细血管充血、出血 ;4种酶的活性在照射后 14d内与对照组比较均有不同程度降低 ,尤其在照射后 7d内显著降低 (P <0 .0 5或P <0 .0 1) ,8× 10 3 V/m和 2× 10 4V/mEMP照射后 ,2 8d内大多数时间点的红细胞数、红细胞压积及血红蛋白浓度明显增加 ,6× 10 4V/mEMP照射后 2 8d ,红细胞数、红细胞压积及血红蛋白浓度显著增加 (P <0 .0 5或P <0 .0 1)。结论 睾丸生精细胞的缺血、缺氧及能量代谢障碍 。
Objective To explore the effect of e lectromagnetic pulse irradiation on energy metabolism of spermatogenic cells in mice. Methods One hundred and fourteen male Kunming mice were randomly divided into a irradiation group (108 rats) and a control gro up(6 rats). The mice in the irradiation group were divided into several subgroup s and irradiated generally for five times by 8×10 3V/m, 2×10 4V/m or 6×10 4V/m electromagnetic pulse (EMP),respectively, within about 2 minutes, each time of irradiation lasted for 20 to 25 seconds. The activity of lactate dehydrogena se (LDH), succinate dehydrogenase (SDH), glutamate dehydrogenase (GDH) and sorbi tol dehydrogenase (SODH), erythrocyte and its parameter, hemoglobin in periphera l blood were observed, and pathological changes of testis were tested at 6h,1d,3 d,7d,14d and 28d after the last irradiation. Results It was shown that EMP irradiation caused striking edema, necrosis and shedding of spermatogenic cells, scarceness and deletion of mature sperm within seminifer ous tubule; edema, accumulation of protein edema fluid, congestion and hemorrhag e of blood capillary within the interstitial could also be observed.In compariso n with the control group, the activities of LDH,SDH, GDH and SODH all decreased to various extend at the time point of 14d after irradiation,and their decrease was most remarkable at the time point of 7d after irradiation( P <0.05 or P <0.01); significant increase in erythrocyte counts, hematocrit values and hemo globin concentration were observed in the mice irradiated generally by 8×10 3V /m and 2×10 4V/m EMP in most of time,and those irradiated by 6×10 4V/m EMP a t the time point of 28d after the irradiation( P <0.05 or P <0.01). Conclusion The results suggested that ischemic hypoxia and disturbance of energy metabolism in spermatogenic cells may play an important r ole in spermatogenic cell injury induced by EMP irradiation.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2003年第9期516-519,共4页
Chinese Journal of Physical Medicine and Rehabilitation
基金
军队总后勤部"十五"指令课题基金资助 (No.0 1L0 2 3)
