摘要
通过检测不同病因引起的胸腔积液患者胸液及血清的干扰素-γ和腺苷脱氨酶(ADA),探讨其对良恶性胸水疾病的鉴别意义.对37例结核性胸膜炎及25例癌性胸液患者和8例漏出液患者,用酶联免疫吸附法(ELISA)检测胸液的干扰素-γ,Giusti’s比色法测定ADA.结果显示结核性胸膜炎患者胸液中的干扰素-γ水平(490.83±384.67)pg/mL,明显高于癌性胸液(36.40±90.85)pg/mL和漏出液组患者胸液(14.87±5.96)pg/mL(P<0.001),且重叠性很小.结核性胸膜炎患者胸液中.ADA明显高于癌性胸液患者(P<0.001).干扰素-γ诊断结核性胸液的敏感性81％,特异性97％,正确率90％,阳性似然比27;ADA诊断结核性胸液的敏感性89％。特异性97％,正确率93％,阳性似然比29.6.提示胸液的干扰素-γ在局部抗分枝杆菌感染中起了一定的作用,检测胸液中干扰素-γ和ADA对结核性胸腔积液和恶性胸腔积液的鉴别诊断有帮助.
This paper discusses the diagnostic significance of interferon 7 (INF-γ) and adenosinase deaminase (ADA) in differential diagnosis of pleural effusions. Levels of INF-y was measured by enzyme-linked immunosorbent assay, and ADA activity was determined by Giusti' s colorimetric method. 37 patients with tuberculous pleural effusions and 25 patients with malignant pleural effusions and 8 patients with pleural transudates were studied. The levels of INF-γ in patients with tuberculous pleural effusions (490.83 ± 384.67 ) pg / mL were elevated over those with malignant pleural effusions (36. 40±90. 85) pg / mL and pleural transudates (14.87±5. 96) pg / mL (P<0.001). The diagnostic sensitivity of the INF-γ for tuberculous pleural effusions is 81 % . Specifity is 97% , and the over accuracy is 90%. The diagnostic efficiency of ADA is as follows: sensitivity 89% , specifity 97% , and the over accuracy 93%. The results show that the INF-γ may play an important role in local anti-mycobacterial infection. The changes of INF-γ and ADA in pleural effusions are of clinically diagnostic value in distinguishing tuberculous from non-tuberculous pleural effusions.
出处
《南京大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第5期590-593,共4页
Journal of Nanjing University（Natural Science）
