摘要
应用反相高效液相色谱 蒸发光散射检测法对安宫牛黄胶囊中的胆酸含量进行了测定。色谱柱用JASCOC18柱(250mm×4 6mmi d ,5μm),流动相为甲醇 0 1%(体积分数)醋酸水溶液(体积比为9∶1),流速1 0mL/min,柱温为室温;蒸发光散射检测器漂移管温度40℃,载气(N2)流速4 0L/min。在上述条件下测得胆酸的进样量为0 50～5 02μg时线性关系良好(r=0 9993),最低检测限达到0 05μg,平均加样回收率为97 1%。该方法快速简捷,精密度高,重现性和准确度良好,可为安宫牛黄胶囊的质量控制提供更为科学的依据。
A method for the determination of cholic acid in AnGong Niuhuang capsule by reversedphase high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) has been established. Cholic acid was successfully separated on a JASCO C18 column (250 mm×46 mm id, 5 μm) at the ambient temperature, using methanol01% acetic acid solution (9∶1, v/v) as mobile phase. The flow rate was 10 mL/min. The drift tube temperature and nitrogen carrier gas flow rate of the ELSD were set at 40 ℃ and 40 L/min respectively. The linear range was 050-502 μg (r=0999?3). The detection limit was 005 μg and the average recovery was 971%. The results of the experiments showed that this method is accurate, rapid, simple and reproducible, and suitable for the analysis of cholic acid. The method is also proposed to be used for the quality control of AnGong Niuhuang capsules.
出处
《色谱》
CAS
CSCD
北大核心
2003年第5期494-496,共3页
Chinese Journal of Chromatography
基金
国家自然科学基金资助项目(20075005)
河北省自然科学基金资助项目(200077
202096).
关键词
反相高效液相色谱
蒸发光散射检测
测定
安宫牛黄胶囊
胆酸
中药
有效成分
high performance liquid chromatography
evaporative light scattering detection
cholic acid
An-Gong Niuhuang capsule
