摘要
本实验用一次性人体胸片(0.118mGy)、胸透(4.920mGy)、胃肠检查(40.425mGy)的X射线剂量,分别一次性均匀照射小鼠,照后7小时观察骨髓细胞染色体畸变率及微核率,结果随剂量的增加染色体畸变率及微核率均增高。中期染色体畸变率与间期细胞微核率两者呈直线相关(r=0.96,P<0.05、直线回归方程为y=—0.32+1.37x)。染色体畸变类型以染色体断片为主。说明在小剂量x射线情况下,引起染色体畸变主要是断片。微核的产生主要由断片形成。所以,通过观察微核率就可得知染色体断裂情况,从而进一步得知此x射线剂量对哺乳动物及人体遗传物质的损伤程度。
The rates of chromosomal aberration and medulla ossium cells micronucleus w(?)re calculated 7 hours after mice were evenly and only once exposed to X-ray with a few dosages, respectively, the racico-photo(0.118mGy),the racico-fluoroscope(4.920mGy)and barium meal examination of gastro-intestine(40.425mGy). The results are that both chromosomal aberration rate and micronucleus one increased along with radiative dosage rising; The linear correlation was found to exist between chromosomal aberration rate at the metaphase mitosis and micronucleus rate at the interphase one(r=0.96,P<0.05,y=-0.32+1.37x). The types of chromosomal aberration mainly shows the chromosomal break fregament. These suggested that the chromosomal break fregament may be the most important reason of chromosomal aberration at the small radiative dosage. The micronucleus primarily evolved from the chromosomal break fregament. We concluded that the chromosomal break degree could be determined through the examination of micronucleus rate,and then the damage degree of hereditary material in the human being and the mammalian resulted from the radiation with this level of dosage is also estimated.
出处
《青海医学院学报》
CAS
1992年第2期76-81,共6页
Journal of Qinghai Medical College
基金
青海省教育厅资助课题
关键词
X线
骨髓
染色体畸变
微核率
X-ray
Medulla ossium cells of mice
Chromosomal aberration Micronucleus rate
