摘要
目的 观察亚砷酸钠诱导体外培养的人皮肤细胞的作用 ,探讨砷性皮肤损伤的分子机制。方法 原代培养皮肤成纤维细胞和表皮细胞 ,通过直接细胞计数法和噻唑蓝 (MTT)还原法检测亚砷酸钠诱导对 2种细胞系生长的影响。结果 成纤维细胞较表皮细胞易于培养 ,与对照组比较 ,不同剂量的亚砷酸钠诱导后 ,吸光度值均有升高 ,有剂量 -效应关系 (P <0 .0 1)。 0 .5～ 8.0 μmol/ L 浓度的亚砷酸钠对成纤维细胞有明显的增殖作用 ;低浓度 (0 .8,1.6 μm ol/ L)对表皮细胞有明显的增殖作用 ,浓度高于 3.2 μm ol/ L 时 ,表皮细胞生长受抑制。 10 μmol/ L亚砷酸钠对皮肤成纤维细胞有明显的毒性作用。结论 无机砷对人皮肤细胞有双向调节的促增殖作用 ,可能是无机砷引起慢性砷中毒皮肤损伤不同表现的一个重要原因 。
Objective To investigate the damage effects of arsenic on primary cultured skin cells induced by inorganic arsenics.Methods Cell number counting, MTT reduction assay,transmission electron microscope were performed in primary cultured skin cells, and sodium arsenite was used as tester inorganic arsenics.Results With different concentration of sodium arsenite 0.5～8.0 μmol/L, the skin fibroblasts were markedly stimulated,and a dose effect relationship was observed. Sodium arsenite at lower concentration( 0.8,1.6 μmol/L) induced mainly degree proliferation of epidermic cell while inhibiting cell growth beyond 3.2 μmol/L.At concentration of 10 μmol/L, however, sodium arsenite induced injury of skin fibroblasts was observed.Conclusions The proliferation of the different skin cell line is regulated in a double phase manner, it may play a critical role in the development of skin injuries caused by inorganic arsenics,and the mechanism of DNA injury in primary cultured human skin cells need to be studied further.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2003年第5期385-386,共2页
Chinese Jouranl of Endemiology
基金
国家自然科学基金
新疆科技厅资助项目(3 99690 0 4)
