摘要
用BALB/C小鼠作为动物模型,应用放射免疫分析法对烧伤后早期小鼠腹腔液申的前列腺素E_2(PGE_2)进行动态测定,同时检测小鼠脾淋巴细胞对刀豆蛋白A刺激的反应性.结果表明,烧伤后7d内各期的PGE_2水平均明显高于正常对照值(67.2±36.8nmol/L,P<0.05～0.001);同时脾淋巴细胞对刀豆蛋白A刺激的反应性在烧伤后明显降低(P<0.05～0.001).体外实验也表明,烧伤鼠腹腔液提取物对正常鼠的淋巴细胞转化功能有抑制作用,与未烧伤鼠的腹腔液提取物的作用相比,具有明显差异(P<0.05).提示烧伤后体内的PGE_2生成明显增多,而且对淋巴细胞转化功能具有一定程度的抑制作用.说明烧伤后早期体内PGE_2的增高是介导免疫功能抑制的因素之一.
Using the BALB/C mice as an animal model, the kinetic changes in the production of Prostaglandin E_2(PGE_2) in the peritoneal fluids of the mice during the early stage of burns, were determined by means of radioimmunoassay. The ConA-induced ~3H-thymidine incorporation in the mice' s splenocytes were examined simultaneously. A series of in vitro tests of PGE_2 were carried out with the extracts obtained from peritoneal fluid of both burned and unburned mice. The results showed that the levels of PGE_2 productionwere elevated markedly after burns, as compared with the control values (P<0.05～0. 001), and the effects of significant inhibition of ConA-induced ~3H-Tdr incorporationcorrelated with the higher PGE_2 levels (P<0.05～0.001). A remarkable difference existed between the immunosuppressive effects of the burned and unburned mice' s peritoneal fluid extracts(P<0.05). The above suggests that the production of PGE_2 is increased
after burns, which is able to alter the lymphocyte function. It could be deduced that the elevated PGE_2 production after burns is one of the mediators to the immunosuppression following thermal injury.
出处
《青岛医学院学报》
1992年第3期179-183,共5页
Acta Academiae Medicinae Qingdao Universitatis
关键词
前列腺素
烧伤
淋巴细胞转化
prostaglandin E
lymphocyte transformations burns
radioimmunoassay
mice
inbred BALB C
