摘要
目的 评价双抗体夹心ELISA法检测尿Ⅳ型胶原(Ⅳ-c)对肾脏疾病患者系膜细胞增殖、细胞外基质分泌和肾小球硬化的意义。方法 比较分析该方法测定15名正常人和51名肾病患者的尿Ⅳ-c。结果 双抗体夹心ELISA法的灵敏度为1.2ng/ml;检测范围3.2～200ng/ml。原发性系膜增殖性肾小球肾炎(MsPGN)组和非MsPGN组尿Ⅳ-c、尿白蛋白(Alb)显著高于正常对照组(P<0.01),MsPGN组尿Ⅳ-c显著高于非MsPGN组(P<0.01)。MsPGN、糖尿病肾病和IgA肾病患者的尿Ⅳ-c明显高于正常对照和其它肾病患者(P<0.05,P<0.01)。结论 双抗体夹心FLISA法检测尿Ⅳ-c简便、灵敏。
Objective To develope enzyme immunoassay for urinary IV collagen in order to evaluate proliferatoin of mesangial cell and accumulation of extracellular matrix and renal fibrosis in nephropa-thy patients. Methods Assay the level of urinary IV collagen (IV - c) , urinary albumin in 15 normal persons was assayed and 51 nephropathy patients. Results The detection limit of this assay was 1.2ng/ml, The working range was 3.2-200ng/ml. The levels of urinary IV - c and urinary albmumin were significantly higher in the membranoproliferative - glomerulonephropathy (MsPGN) group and the non - MsPGN group than normal controls (P <0.01) , The urinary IV - c was significantly higher in the MsPGN group than the non - MsPGN group (P < 0. 01 ). The levels of urinary IV - c were much higher in diabetic nephropathy ( DN) and IgA nephropathy ( IgAN ) patients than the normal group and the other nephropathy group (P <0. 05 , P < 0. 01). Conclusion We have developed a sensitive and simple ELISA for urinary IV -c.
出处
《上海第二医科大学学报》
CSCD
2003年第5期403-405,共3页
Acta Universitatis Medicinalis Secondae Shanghai
基金
上海市卫生局青年课题资助项目(4Y7)
