摘要
目的:研究重组分泌型内皮抑素(endostatin)对兔角膜新生血管的抑制作用。方法:利用角膜缝线法诱导兔角膜新生血管形成。在缝线后第1天,将重组内皮抑素和生理盐水分别注射于兔球结膜下,观察角膜新生血管生长状况及组织病理学改变。结果:重组内皮抑素治疗组新生血管长度及生长面积明显少于对照组,差异有统计学意义(P<0.05);光镜下,治疗组与对照组比较,基质层新生血管少,管腔小,浆细胞及成纤维细胞少。结论:球结膜下注射重组内皮抑素可有效抑制兔角膜新生血管的形成,为临床上角膜新生血管的防治提供新的方法。
Objective:To study the inhibition effects of recombinant human secretory endostatin on corneal neovascularization of rabbits.Methods:Stitching cornea was used to induce the formation of corneal neovascularization in rabbits.On the first day after manipulation,recombinant endostatin and nor-mal saline were separatively injected into subconjunctival tissues to observe the growth of corneal neovas-cularization and histopathological changes.Results:Endostatin treatment group showed shorter elongations and smaller areas of new blood vessels than normal saline group.Moreover,fewer neovascularities,plasmo-cytes and fibroblasts in endostatin treatment group were observed under the light microscope(P<0.05).Con-clusion:The results indicated that the subconjunctival injection of recombinant endostatin could inhibit the growth of corneal neovascularization,which will provide a new method for the prevention and treatment of corneal neovascularization in clinic.
出处
《山东大学学报（医学版）》
CAS
2003年第4期348-350,共3页
Journal of Shandong University:Health Sciences
基金
国家自然科学基金资助项目(30100078)
山东省卫生厅资助项目(2001CA2CAA1)
