摘要
目的探讨在大鼠角膜基质缝线诱发角膜新生血管(CNV)模型的制作技巧及特点。方法20只SD大鼠,在手术显微镜下用10-0号尼龙线铲针在角膜基质层间置3针缝线(11点、12点、1点3个钟点位置),自距离角膜缘略小于2.0mm向瞳孔中心方向进针。采用裂隙灯显微数字图像处理系统动态观察角膜新生血管的生长情况,并观察CNV模型的角膜组织病理学变化。结果①角膜缝线后4d可见明显从角膜缘伸入角膜的毛刷状小血管、垂直角膜缘切线方向,角膜缝线处水肿;随着角膜水肿继续加重,8d新生血管延伸到达或超过缝线位置,分支密集并互相吻合形成袢状血管网、生长范围不超过50%圆周;②HE染色显示在正常角膜切片基质层仅见成纤维细胞和纤维母细胞,而在CNV模型角膜切片则见角膜上皮层水肿增厚、浅层基质内密集大量新生血管、管腔大小不等,新生血管周围有大量分叶核炎性细胞浸润。结论大鼠角膜显微缝线诱导的新生血管生长稳定、适于定量研究。
AIM: To explore the skills and characteristics of constructing corneal neovascular (CNV) model in rats with the induce of corneal stitch.· METHODS: For 20 SD rats, 10-0 nylon needle was used to stitch on the corneal stroma. The 3 stitches were on the sites of 11 o'clock, 12 o'clock and 1 o'clock. The growth and pathological change of CNV was observed with slit lamp micro-digital image process system.· RESULTS: ①Neovascular appeared probing from limbus corneae into cornea 4d after stitching. The stitch sites were edematous. The neovascular reached to stitch sites at 8d, and then vascular net formed. ②HE staining indication: only fibroblast and fibrocyte could be seen in normal corneal section, but in CNV model corneal section, there were increased edema at corneal epithelia, neovascular of different sizes in sublimis matrix and inflammatory cellular infiltration around neovascular.· CONCLUSION: Corneal neovascular induced by corneal stitch in rats grows steadily, and is suitable for quantitative researches.·
出处
《国际眼科杂志》
CAS
2004年第5期820-823,共4页
International Eye Science
