摘要
作者对体外培养的人牙龈成纤维细胞（ＧＦ）和牙周韧带细胞（ＰＤＬＣ）采用免疫组化ＡＢＣ染色及显微分光光度法分析，测定ＧＦ和ＰＤＬＣ中前列腺素Ｅ２（ＰＧＥ２）、６－酮一前列腺素Ｆ１ａ．（６－Ｋ－ＰＧＦ１ａ）和血栓素Ｂ２（ＴＸＢ２）含量，以探讨其与牙周炎的关系，结果表明ＧＦ的ＰＧＥ２吸光度为０．２５±０．０３，６－Ｋ－ＰＧＦ１ａ的吸光度为０．２０±０．０３，与对照组相比均有非常显著性差异；ＰＤＬＣ的吸光度分别为ＰＧＥ２０．２１±０．０２、ＴＸＢ２０．１６±０．０３和６－Ｋ－ＰＧＦ１ａ０．１３±０．０２，与对照组相比均有非常显著性差异。表明ＧＦ和ＰＤＬＣ均可分泌一定量的ＰＧＥ２、ＴＸＢ２和６－Ｋ－ＰＧＦ１ａ，但分泌的量略有差异，提示ＧＦ和ＰＤＬＣ可通过分泌ＰＧ，影响牙龈炎症变化和牙周组织再生。
This investigation analyses the contents of prostaglandin E2(PGE2) 6-ketoprostaglandin F1a (6-k-PGF1a)and thromboxane B2(TXB2) in gingival fibroblast(GF) and periodontal ligament cell(PDLC) by microspectrophotometer. The results showed that the absorbance of PGE2 in GF was 0. 25±0. 03, the absorbance of 6-k-PGF1a was 0.20±0. 03, there were significant difference between PGE2 6-k-PGF1a and control group.The absorbance of PGE2. TXB2 and 6-k-PGF1a and control group. The absorbance of PGE2. TXB2 and 6-k-PGF1a in PDLC each were 0. 20±0. 02、0. 16±0. 03 and 0.13±0.02. They were much bigger than control group(0. 07±0.01 ), which indicated prostaglandins in GF and PDLC can affect guided tissue regeneration and gingivitis degree, and there were important relationship between PG. in GF.PDLC and preiodontitis.
出处
《上海口腔医学》
CAS
CSCD
1995年第3期155-157,共3页
Shanghai Journal of Stomatology
