摘要
对甜樱桃品种"拉宾斯"和"斯特拉"种胚的离体培养进行了研究。基本培养基为1/2MS,蔗糖浓度为3%。结果表明,甜樱桃胚培养的最佳取样时期为盛花后45d(PF1=0.9)。60和80d的低温(2～4℃)处理对于打破胚休眠效果较好。在不进行低温处理的条件下,GA3(15mg/L)对打破带种皮和不带种皮胚的休眠有作用,GA3(10mg/L,5mg/L)与BA(2mg/L,4mg/L)同时使用对打破胚的休眠更为有效。在去种皮的条件下,GA3和BA打破胚休眠的作用更为明显。
The study was carried out on embryo culture of 'Lapins' and 'Stella' of sweet cherry(Prunus avium L.)using 1/2MS basal medium with 3% sucrose. The result showed that 45 days of fruit growth after full-bloom (PF1 was about 0.9) was the best period for embryo culture. 60 and 80 days of chilling treatments(2~4℃) was suitable for breaking embryo dormancy. GA3 (15 mg/L) was effective for breaking the embryo dormancy whether the embryo were with or without coat. Higher plant stands were found on the media containing both GA3 (10mg/L, 5mg/L) and BA.(2mg/L, 4mg/L). GA3 and BA were more effective in breaking embryo dormancy when naked embryos were used.
出处
《莱阳农学院学报》
2003年第3期162-164,共3页
Journal of Laiyang Agricultural College
关键词
甜樱桃
胚培养
低温处理
离体培养
embryo culture
sweet cherry
chilling treatment
GA_3
BA
