摘要
目的 探讨苯丙酸诺龙对烧伤后成纤维细胞 α1( )型前胶原蛋白 m RNA表达的影响及其作用机制。 方法 Wistar大鼠 32只 ,制成 2 0 % TBSA深 度烧伤模型 ,随机分为苯丙酸诺龙治疗 (NP)组和对照组 ,于 4、7、14和2 1天分别取创面肉芽组织 ,测定其成纤维细胞中雄激素受体及 α1( )型前胶原蛋白 m RNA含量 ,分析比较两组间成纤维细胞雄激素受体和α1( )型前胶原蛋白 m RNA的表达及其相关关系。 结果 NP组在 7、14和 2 1天α1( )型前胶原蛋白 m RNA的表达均高于同期对照组 ,两组间比较有统计学意义 (P<0 .0 5 )。 NP在 4、7、14和 2 1天成纤维细胞雄激素受体的表达均明显高于同期对照组 ,有统计学意义 (P<0 .0 5 )。雄激素受体的表达与胶原蛋白基因的表达之间有相关关系 ,且有统计学意义。 结论 NP能促进 型前胶原蛋白 m RNA和成纤维细胞雄激素受体的表达 ,二者间存在正相关关系 ,NP在转录水平促进胶原蛋白的合成 ,这种作用与改变成纤维细胞雄激素受体的含量有关。
Objective To explore the effects of nandrolone phenylpropionate (NP) on the expression level of proα1(Ⅰ) collagen after burn in rats and the possible mechanism involved in the process. Methods Thirty two Wistar rats with a deep second degree scald injury and 20% of total body surface area were randomly divided into two groups to receive either 5 mg/kg NP(NP group) or normal saline (control group) every other day. We analyzed the mean integrated optical density(mIOD) of androgen receptor (AR) to determine the distribution and expression of AR in fibroblasts by immunohistochemistry, and measured expression level of proα1(Ⅰ) collagen mRNA by quantitative fluorescent RT PCR to find the relation between expressions of AR and proα1 (Ⅰ) collagen mRNA. The total specimens were obtained from the scalded rats after 4, 7, 14 and 21 of after burn. Results The expression of proα1 (Ⅰ) collagen mRNA in NP group was significantly higher than that in control group on the 7th,14th and 21st days( P< 0.05), but there was no significant difference on the 4th day. The density of AR in fibroblasts had significant difference ( P< 0.05) between the two groups after 4, 7,14 and 21 days. A positive relationship existed between the expression of proα1 (Ⅰ) collagen mRNA and quantity of AR in fibroblasts(r=0.836). Conclusion The nandrolone phenylpropionate increased the expression of proα1 (Ⅰ) collagen mRNA and enhanced the density of AR in fibroblasts. The higher expression of proα1 (Ⅰ) collagen mRNA had a relation with the change of quantity of AR in fibroblasts.
出处
《中国修复重建外科杂志》
CAS
CSCD
2003年第5期382-385,共4页
Chinese Journal of Reparative and Reconstructive Surgery
