摘要
目的 :研究核因子kappaB(NF κB)在佐剂关节炎 (AA)大鼠滑膜组织中的表达 ,探讨类风湿关节炎(RA)的发病机制。方法 :分别用免疫组织化学法及原位杂交法检测AA大鼠滑膜组织中NF κB蛋白及NF κBmRNA的表达。用显微图像分析系统对结果进行定量分析。结果 :AA大鼠滑膜中NF κB的蛋白及mRNA水平均较正常组明显升高 (P <0 .0 1,P <0 .0 1) ,NF κB蛋白主要分布于滑膜衬里细胞、巨噬细胞、成纤维细胞及淋巴细胞中。胞浆及胞核内均有染色 ,但胞核染色较深 ,多呈团块状分布 ,而胞浆染色较浅。NF κBmRNA阳性细胞与蛋白阳性的细胞种类相同 ,主要分布于胞浆中。结论 :滑膜组织的NF
Objective: Our aims were to study expression of nuclear factor-kappa B (NF-κB) in the synovium of rats with adjuvant arthritis (AA) , and to investigate the pathogenesis of rheumatoid arthritis (RA). Methods: We determined p65 protein with immunohistochemistry method and p65 mRNA with in situ hybridization. Results: In AA group, the levels of p65 protein and p65 mRNA were much higher than those of healthy controls (P<0.01), and p65 expressed mainly in the synovial lining cells, macrophages, fibroblasts, and lymphocytes. Conclusion: The NF-κB in the synovium may be involved in the pathogenesis of RA. This study provides the theoretical basis for the treatment of RA using the inhibitor of NF-κB.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2003年第5期385-387,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目 (3 0 0 70 3 3 0 )
辽宁省教育厅科研基金资助项目 (2 0 12 10 3 2 )
