摘要
为了研究叉头框 c2 (ForkheadBoxc2 ,Foxc2 )基因在心血管发生和发育中的作用 ,通过制作小鼠的Foxc2基因无效突变 ,解析该基因缺失鼠主动脉弓的异常发育状况 .纯合子胎鼠从 12 5天胚胎 (embryo ,E)开始有宫内死亡 ;即使完成宫内发育过程 ,新生鼠出生 2 4h后也全部死亡 .这些鼠全部表现出与人的先天性心血管发育缺陷相似的B型或C型主动脉弓离断 .杂合子鼠发育正常 .E10 5胚胎的原位杂交分析显示 ,Foxc2mRNA在第三、第四和第六弓型动脉强烈表达 ,而第四弓型动脉在E10 5胚胎后逐渐消失 .这些结果表明 ,在主动脉弓形成过程中 。
In order to investigate the potential roles of Forkhead Box c2 (Fox c2) in cardiovascular development, mice lacking Fox c2 locus were produced by targeted mutation and the developmental anomalies in the aortic arch were found. Mice homozygotes for the mutation (Fox c2(-/-)) died embryonically from E12.5 (embryo days, E) to term. Although some of the homozygous mutants were born with abnormalities of the aortic arch, all of them died within 24 h after birth. Fox c2(-/-) homozygous mutants all displayed the Type B or Type C of interrupted aortic arch, which is the same as human congenital cardiovascular anomalies. Mice heterozygous for the mutation developed normally. In situ hybridization analysis on E10.5 embryos showed that Fox c2 mRNA expressed at the third, fourth and sixth arch arteries strongly. However, left fourth arch arteries disappeared at E12.5 gradually. These results suggest that the Fox c2 plays indispensable role in the remodeling of left fourth arch arteries during the formation of aortic arch.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2003年第5期726-731,共6页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目 ( 3 0 2 713 69)
吉林省科技发展项目 ( 2 0 0 3 0 5 3 8 3 )~~
