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高碱性pH诱导紫膜片层表面结构变化的原子力显微镜直接观测 被引量：3

The Direct Observation on The Surface Structure Change of Purple Membrane Induced by High pH Using Atom Force Microscope
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摘要 报道了在高碱性pH下 ,紫膜中细菌视紫红质 (BR)表面结构变化的直观信息 .紫外可见光谱实验发现 ,当pH上升到 12 6 ,BR分子上的生色团视黄醛脱落 ,分子完全变性 ;原子力显微镜实验观测到在此pH下 ,紫膜片层的晶格结构瓦解 ,BR分子在紫膜上无规则聚集 ,同时出现非特征“岛屿”结构和特征“岛屿” The direct information of the surface structure change of purple membrane under high pH was obtained. The UV-VIS spectra showed that BR was totally denatured and lost its retinal under the pH 12.6. It was found by atom force microscope that the crystalline lattice of purple membrane was broken down at this pH. The typical and non-typical 'Island' structures emerged as the BR molecules assemble irregularly in purple membrane.
作者 陈德亮 李辉 韩宝善 徐兵 胡坤生
机构地区 中国科学院生物物理研究所 中国科学院物理研究所
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2003年第5期711-714,共4页 Progress In Biochemistry and Biophysics
基金 国家自然科学基金资助项目 ( 3 0 170 2 3 5 ) 中国科学院重大项目(KJCX1 SW ) 国家重点基础研究发展规划资助项目 ( 973 )(G19980 1 0 10 2 )~~
关键词 高碱性pH 诱导 紫膜 表面结构 原子力显微镜 细菌视紫红质 质子传递蛋白 bacteriorhodopsin purple membrane pH 'Island' structure atom force microscope
分类号 Q936 [生物学—微生物学] Q6-33 [生物学—生物物理学]
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参考文献13

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同被引文献45

  • 1黄力 刘坚 明明 等.细菌视紫红质蛋白在脂质体上的体外定向自组装[A]..高分子液晶态与超分子有序态研究进展[M].武汉:华中科技大学出版社,2002.157-161.
  • 2Atkinson G H,Ujj L,Zhou Yidong. J Phys Chem A,2000,104 (18):4130.
  • 3Liang Bing,Li liqian,Li Meiling,et al. Proceedings of SPIE- The International Society for Optical Engineering, 2000,4104: 64.
  • 4Lensu Lasse, Frydrych Michael, Parkkinen Jussi, et al. Optical Materials, 2004,27(1) : 57.
  • 5Koyama Koichi, Miyasaka Tsutomu. Electrochemistry,2004,72(1) :2.
  • 6Koyama Koichi, Miyasaka Tsutomu. Biochemistry, 2001,53(1): 111.
  • 7Peralvarez-Marin,Alex,Marquez Mercedes,et al . J Biological Chemistry, 2004,279 (16): 16403.
  • 8Facciotti Marc T,Cheunq Vincent S, Lunde Christopheer S,et al. Biochemistry, 2004,43 (17): 4934.
  • 9Jussila Tapio,Li Meiling, Tkachenko Nikolai V. Biosensors and Bioelectronics, 2002,17(6-7): 509.
  • 10Atkinson G H, Zhou Y,Ujj L,et al. J Phys Chem A,2002,106(14):3325.

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生物化学与生物物理进展
2003年 第5期

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