摘要
采用基因枪方法将适用于高等植物的绿色荧光蛋白基因mgfp4转化到水稻愈伤组织之中 ,获得高效瞬时表达 .在荧光显微镜和激光共聚焦显微镜下观察到强烈绿色荧光 .绿色荧光蛋白mGFP4生色团形成迅速 ,转化 4h后就能观察到绿色荧光 ,并且持续时间较长 ,2d后才基本消退 .Southern杂交和卡那霉素 (Kan)选择实验结果表明 ,mgfp4确已得到转化 .以上结果表明水稻愈伤组织适宜于mGFP4的转化和表达 ,为今后构建GFP融合蛋白和分析相关蛋白的生物学功能奠定了基础 .
High-level expression of mGFP4, a GFP variant suitable for higher plants, is obtained through bombardment transformation into rice calli. Bright green fluorescence is visualized by fluorescence and confocal microscopy. The formation of mGFP4 chromophore is swift and green fluorescence is visualized within 4 h following bombardment. Also, the fluorescence emission lasts for about 2 d. The transformation and expression of mGFP4 are demonstrated by Sou thern blotting and Kanamycin selection experiments. Taken together, rice calli are suitable materials for the transformation and expression of mGFP4, and the success of mGFP4 transformation into rice calli will be valuable to our future research in fusing proteins to GFP and their function analysis in rice cells.
出处
《北京师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第3期385-389,共5页
Journal of Beijing Normal University(Natural Science)
基金
国家"九七三"计划资助项目
国家自然科学基金资助项目!(39770 0 77)
北京师范大学青年科学基金资助项目
关键词
绿色荧光蛋白基因
水稻
愈伤组织
瞬时表达
green fluorescent protein
mgfp4
rice calli
microprojectile bombardment
