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人源细粒棘球蚴囊液囊壁及原头蚴抗原在包虫病免疫诊断中的应用

Comparative Evaluation of Antigens from Human Echinococcus granulosus Ilydatid Cyst Fluid,Cyst Membrane & Protoscolex in Immunodiagnosis of Hydatidosis
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摘要 用人源细粒棘球蚴囊液、囊壁及原头蚴抗原对50例包虫病患者,59例正常人及46例非包虫病患者的血清进行酶联免疫吸附试验(ELISA),以对比三种抗原在包虫病免疫诊断实验中的敏感性和特异性。结果显示:囊液、囊壁及原头蚴三种抗原的阳性符合率分别为90%,76%和78%;特异性为96.1%,92.2%和92.2%。经统计学处理,在敏感性及特异性之间均无显著性差异(P>0.05)。这提示,除棘球蚴液外,囊壁及原头蚴也可作为抗原用于 ELISA 进行包虫病的免疫诊断。 Human Echinococcus granulosus hydatid cystfluid(EgHCF),cyst me- mbrane(EgCM)and protoscolex(EgPS) were used as antigens to test the sera from 50 hydatidosis patients,59 hea- lthy subiects and 46 patients with oth- er diseases by ELISA.The result show- ed that the positive rates of EgHCF, EgCM,and EgPS were 90%,76%,& 78%,respectively,and theirs specifi- city being 96.1%,92.2% &92.2%,respec- tively.Statistically,there were no significant differences of sensitivity/ speciality among the three antigens. It is suggested that besides EgHCF, EgCM or EgPS can also be used as the antigens for immunodiagnosis of hydatidosis.
作者 黄俭 张京元
出处 《宁夏医学杂志》 CAS 1992年第1期12-14,共3页 Ningxia Medical Journal
关键词 棘球蚴病 诊断 免疫 ELISA Echinococcosis/diag Enzyme-linked immunosorbent assay Cysts hydatid/diag use Comparative study
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