摘要
为减小电极反应和电极距离过大对于两相电泳的影响,设计了微小型多通道两相电泳设备,并以苯丙氨酸为对象开展了双水相电泳的研究工作。较系统地研究了时间、电流密度、料液初始浓度等因素对葡聚糖-聚乙二醇-水体系中的苯丙氨酸上下相浓度比、萃取率、总传质量以及电流效率的影响。基于分析,给出了计算最大总传质量的半经验式。
In order to overcome the influences of electrode reactions and the long distance between two electrodes on two-phase electrophoresis, a mini-multi-channel aqueous two-phase electrophoresis (mini-MATPE) equipment was designed, and the separation of phenylalanine in this equipment was investigated with dextran-polyethylene glycol-water as a working system. The influences of time, current intensity, initial solute concentration on two-phase concentration ratio, recovery ratio, total mass flux, and electric current efficiency was discussed. Based on the study, a semi-empirical correlation for predicting the maximum mass flux is suggested.
出处
《过程工程学报》
CAS
CSCD
北大核心
2003年第4期317-322,共6页
The Chinese Journal of Process Engineering
基金
国家自然科学基金(编号:29476243)
