摘要
采用柱前衍生反相高效液相色谱法(RP HPLC)测定了江苏地产药材野马追茎叶及其花蕾中的总氨基酸和游离氨基酸。用邻苯二甲醛(OPA)与一氯甲酸芴甲酯(FMOC)联用全自动柱前衍生化反相高效液相色谱仪,liozosphezeC18柱,pH=7.2的醋酸钠缓冲溶液和甲醇为流动相,流速为1.0mL/min,荧光检测器,波长为Ex=340nm、Em=450nm,样品中的氨基酸在30min内得到分离和检测。结果表明:野马追茎叶及其花蕾中含17种氨基酸,游离氨基酸17种,总氨基酸含量分别为2.40%和6.51%,游离氨基酸含量分别0.185%和0.508%,半胱氨酸(cys)和谷氨酸(glu)含量较高,其中7种为人体必需氨基酸。
The contents of the free and combined amino acids in stems,leaves and flowers of Eupatorium lindleyanum DC.in Jiangsu Province are determined by reversedphase high performance liquid chromatography in this study,using precolumn derivatization with ophthaldialdehyde(OPA) and fluorenylmethyl chloroformate(FMOC) RPHPLC,Liozospheze C18 column,the mobile phase including sodium acetate buffer at pH 7.2 and methanol,flow speed of 1.0 mL/min,fluorescence detector(FLD) at wavelengths Ex=340 nm and Em=450 nm,detection time 30 min.The results showed that there are 17 kinds of free and combined amino acids.The contents of combined amino acids are 2.40% and 6.51% in Eupatorium lindleyanum DC.stems and leaves and their flowers,respectively.The contents of free amino acids are 0.185% and 0.508%.The contents of cystatin acid(Cys) and glutamic acid(Glu) are much higher than other amino acids.And among them there are 7 kinds of human essential amino acids.
出处
《林产化工通讯》
2003年第5期7-9,共3页
Journal of Chemical Industry of Forest Products
关键词
柱前衍生化
RP—HPLC
测定
中药
野马追
氨基酸
Eupatorium lindleyanum DC.
pre-column derivatization
combined amino acids
free amino acids
