摘要
目的 :探讨自身免疫反应在急性心肌梗死 (AMI)后心肌损伤中的作用。方法 :复制AMI大鼠模型和假手术作对照 ,6周后将 10 0× 10 6 ～ 15 0× 10 6 脾细胞过继转输给同源近交大鼠 ;4周后检测受者大鼠血流动力学、组织病理和抗肌球蛋白重链 (MHC)抗体。结果 :AMI大鼠和转输大鼠 (AMI T组 ) (8 2 2只 )血清中检测到抗MHC抗体 ,假手术大鼠和转输大鼠(Sham T组 ) (0 2 0只 )均为阴性 (P <0 0 5 ) ;AMI T组 (n =10 )大鼠发生心脏特异性炎症反应和心功能受损 ,Sham T组心脏无异常变化。结论 :AMI T大鼠发生了淋巴细胞介导心肌损伤和心功能下降 ,心肌自身免疫反应可能是AMI后心室重塑的新颖机制。
Objective:To study the autoimmune response against self myocardial tissue in an experimental rats model of acute myocardial infarction (AMI) and reveal a potential role of autoimmune mediated myocardial injury involved in ventricular remodeling after AMI.Methods:An experimental animal model of AMI was adopted by in vivo ligation of left anterior descending branch (LAD) in Wistar rats After six weeks, spleens were removed and splenocytes were collected About 100×10 6～150×10 6 splenocytes were freshly transferred to syngeneic inbred rats Four weeks later, these recipient rats were anesthetized for hemodynamics analysis by catheter technique Antibody against cardiac myosin heavy chain (MHC) was screened by enzyme linked immunosorbent assay Histopathological studies were performed on all hearts Results:The antibody against cardiac MHC was positive in 8 of 22 AMI rats and recipient rats, and in 0 of 20 sham operation and recipient rats,P<0 05 The specific inflammation of the myocardium was found in recipient rats and non in sham transfer rats The impairment of left ventricular function was shown in recipient rats (n=10), which was characterized by slight decline of +dp/dtmax ( 1 323 ±55 kPa/s vs 1 418 ±82 kPa/s,P<0 05) Conclusion:The findings showed in vivo evidence of lymphocytes mediated myocardial injury and cardiac function impairment by adoptive transfer of freshly prepared splenocytes from AMI rats, which indicated a possible novel mechanism of autoimmune mediated myocardial impairment involved in ventricular remodeling after AMI. [
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2003年第9期642-644,共3页
Chinese Journal of Immunology
基金
卫生部临床学科重点项目 (No 970 70 2 3 8)
高等学校骨干教师资助计划 (No 教技司 2 0 0 0 65 )
