摘要
对猪下丘脑混合神经细胞的取材、分离及培养进行了研究。结果表明:培养猪下丘脑混合神经细胞 应取材于60日龄以内的胎猪,采用0.125％的胰酶作用25 min,42～45日龄胎猪下丘脑神经细胞分散和培养 效果较好。
Sampling, isolation and culture of mixed hypothalamus nerve cell were primarily studied. The results indicated that suitable sampling period for hypothalamus cell from pig fetus would be within 60 days of pregnancy and treated with 0.125 % poncrease for 25 min, with 42 to 45 days being the best.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2003年第4期439-441,共3页
Journal of Jilin Agricultural University
基金
国家自然科学基金资助项目(30170713)
