摘要
本实验用改良的有刻度的毛细管收集龈沟液,用单扩法测定了18例慢性成人型牙周炎患者(AP)龈沟液和血清的 IgG、IgA 含量,同时做了取样牙位牙龈的免疫组化检查。结果:龈沟液 IgG、IgA 含量分别为7.08±2.53g/L、780±160mg/L,分别占血清的69%和57%。牙龈免疫组化检查结果:牙龈中以 IgA 阳性浆细胞为主,IgG、IgM 细胞较少。提示:AP 患者在牙龈炎症明显时,局部牙龈以 IgA 浆细胞为主,而龈沟液中 IgG 的含量较高,两者无一致性关系。笔者认为:龈沟液中 IgA 含量较低可能与牙周袋内某些病原菌使 IgA 裂解有关,龈沟液中的 IgG 主要来自血清。
The livels of IgG,IgA in gingival crevicular fluid and serum were measured by immunodiffusion method.Gingival crevicular fluid was collected in improved and graduated capillaries from 18 adult periodontitis patients,at the same time,the pocket walls were cut for immunohistochemical study.The result showed that the levels of IgG,IgA were 708±253mg/100ml 78±16mg/100ml and the percent of gingival crevicular fluid to serum were IgG 69%,IgA 57%.Immunohistochemical study indicated that most of the cells in pocket walls were the IgA positive plasma cells.This study suggests that the majority of Ig synthesized in gingive are probably IgA.The levels of IgA are lower in gingival crevicular fluid,which may Le the result of cleavage of IgA by some bacteria.Most of IgG in gingival crevicular fluid comes from serum.
关键词
牙周炎
龈沟液
免疫球蛋白
periodonititis
gingival cervicular fluid
IgA
IgG
