摘要
证实了Wetter和Youngs硫脲紫外法用于中国菜籽饼粉(包括未脱毒和用双液相萃取脱毒饼粉)总硫甙测定是合适的,其最小检出量以丁烯基异硫氰酸酯计可达0.3～0.4 mg/g。对硫甙含量不是太小的样品用白芥子粉替代芥子酶可大大简化手续。宁油七号菜籽饼粉最适宜测定波长为241nm,此时校正光密度与硫甙含量(mg/g)间的换算因子用酶时为2.5～2.6,用青海白芥子粉时为3.0～3.1,而用245nm校正光密度则相应为3.2～3.5和3.9～4.0。
It has been proved that the thiourea-UV method proposed by Wetter an Youngs is suitable for determination of total glucosinolate content in Chinese rapeseed meals including the detoxic meals produced by two-phase processing. The detection limit is 0.3～0. 4 mg/g meal (expressed as 3-butenylisothiocyanate ). To facilitate the determination, it is desirable for meals with not too low glucosinolate content to use mustard seed directly as a substitute for myrosinase. The most suitable determination wavelength is 241 nm for Ninyou～#7 rapeseed. The conversion factors for conversion of corrected optical density OD_(241)~C to glucosinolate content (mg/g) are 2.5～2. 6 when enzyme is used, and 3.0～3.1 when Qinghai mustard is used. For OD_(245)~C , the conversion factors change to 3. 2～ 3.5 and 3.9～4.0 respectively.
关键词
比色法
硫代葡萄糖甙
菜籽饼粕
glucosinolates, ultraviolet spectrophotometry, rapeseed meal.
