摘要
目的 探讨环氧化物水解酶 (EPHX)和谷胱甘肽转硫酶 (GST)基因型及血清黄曲霉素B1(AFB1)加成物含量和肝癌易感性的相关性研究。方法 以 3对肝癌高发家族 (6 2例 )和相对应的非癌对照家族成员 (5 8例 )为研究对象 ,分别采用放射免疫法 ,PCR法测定所有成员血清中AFB1 白蛋白加成物量、HBsAg及血细胞的EPHX ,GSTT1,GSTM1的基因型。结果 1.高发家族成员有 4 1.9% (2 6 / 6 2 )血清AFB1 白蛋白加成物含量高于所有被测成员的中位值 ,而对照家族仅有 15 .5 % (9/ 5 8)高于中位值 ,二组之间呈显著性差异 (P <0 .0 0 1)。2 .在对照家族和高发家族AFB1 白蛋白含量低于中位值的人群中GSTM 1,GSTT1,EPHX基因型的分布未见明显的差异 ;而AFB1 白蛋白含量高于中位值的高发家族人群 ,其EPHX 113编码位突变型百分率明显高于对照家族 (P <0 .0 0 1) ,但GSTT1,GSTM 1的基因型的分布无显著性差异。结论 EPHX基因 113编密子的突变与机体AFB1暴露后形成的加成物的含量增加有关 ,从而可以推断与个体对黄曲霉素的敏感性和肝癌的易感性相关。
Objective To study the association between the Aflatoxin B_1(AFB_1) metabolism enzyme GST and EPHX polymorphism and the susceptibility to hepatocellular carcinoma(HCC). Methods To the 62 cases from HCC high risk families and 58 from matched control families, the polymorphism of GSTM1, GSTT1 and EPHX in blood cell DNA was tested by PCR and the AFB_1-albumin-adducts and HbsAg in serum by radio-immunoassay. Results 1. Among the high risk family group and control group, 41.9% (26 /62) and 26% (9 /58) respectively showed an AFB_1-albumin adducts level higher than the average median level., the difference was significant ( P <0.001). 2. Significant difference of frequency of EPHX code 113 mutant type was observed between high risk family group and matched control family group( P < 0.001). There were no any other difference in other groups and other genes. Conclusion The mutant of EPHX code 113 may increase the risk of AFB_1 exposure to human beings and their susceptibility to hepatocellular carcinoma.
出处
《肿瘤》
CAS
CSCD
北大核心
2003年第4期287-289,共3页
Tumor
基金
九五国家医学科技攻关项目资助 (编号 :85 914 0 3 0 3 )
