摘要
目的研究细胞色素P450(CYP)1A1和谷胱甘肽转硫酶M1(GSTM1)和T1(GSTT1)基因多态性与食管癌易感性的关系。方法应用PCR-RFLP技术对87例食管癌患者和162例无上消化道肿瘤的健康者的CYP1A1、GSTM1和GSTT1的基因多态性进行分析。比较两组基因型频率的差异。结果食管癌组CYP1A1 Ile-Val多态位点各等位基因和基因频率与对照组比较,差别有统计学意义,其中Val/Val基因型在食管癌组的频率(29.9%)显著高于对照组(13.0%)(χ2=10.54,P<0.01),OR值为3.10,95%CI为(1.57,6.14),而CYP1A1的Ile/Val、Ile/Ile多态位点和GSTM1与GSTT1的缺失多态性的基因型频率与对照组比较,差别无统计学意义(P>0.05)。结论CYP1A1 Val/Val基因型为食管癌的重要易感因素之一,而GSTM1与GSTT1的基因型可能与食管癌的发生无关。
Objective To study the possible relationship between CYP1A1, GSTM1 and GSTrl genetic polymorphisms and the susceptibility of esophageal carcinoma. Methods Using case - control study, the CYP1 A1, GSTM1 and GSTrl genetic polymorphisms of 87 cases of esophageal carcinoma and 162 healthy cases were genotyped by PCR - RFLP techniques. Compare the genotypic frequency between the two groups. Results In esophageal carcinoma group the CYP1A1 Val/Val genotype frequency is 29. 89%, significantly higher than in the control group （ 12. 35% ） （ X^2 = 10. 54, P 〈 0. 01）. No significant difference of the frequencies of CYP1 A1 Ile/Val, CYP1 A1 Ⅱe/Ⅱe, GSTM1 and GSTrl genotypes were found between the two groups. Conclusion It is demonstrated that the CYP1A1 Val/Val genotype is an important factors for susceptibility of human esophageal carcinoma and the genotype of GSTM1 and GSTrl possibly has no significant relationship with human esophageal carcinoma.
出处
《实用心脑肺血管病杂志》
2008年第7期16-17,共2页
Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease
