摘要
目的 研究全反式视黄酸 (atRA)对大鼠自体移植静脉内膜平滑肌细胞 (VSMC)增生与凋亡的影响。方法 建立 40只大鼠自体静脉移植模型 ,随机分成atRA组和对照组 ,每组按 7、14d随机分成 2个亚组 ,每只大鼠分别于术前 4d至术后 10d进行a tRA( 10mg·kg-1·d-1)或相同剂量的溶剂 (植物油 )喂养 ,规定时间点取材固定 ,Masson染色 ,免疫组化测定增殖细胞核抗原 (PCNA)及TUNEL法检测平滑肌细胞凋亡 ,计算机图象分析仪测量移植血管内膜厚度、计算内膜增生指数及细胞增殖和凋亡百分比。结果 术后 7或 14datRA组移植静脉段的内膜厚度、增生指数及VSMC增殖指数均明显低于对照组 (P <0 0 5 ) .而凋亡指数则明显高于对照组 ( P <0 0 5 )。结论 全反式视黄酸 (atRA)可以抑制自体移植静脉内膜增生及平滑肌细胞增生 。
Objective To examine the effect of all-trans-retinoic acid (atRA) on proliferation and apoptosis rates of smooth muscle cells in healing vein bypass grafts.Methods Autogenous vein graft model was established in 40 rats by transplanting the internal branch of the jugular vein to the carotid by end-to-end anastomosis.Animals were divided at random into two groups:atRA group and control group.The animals received atRA(10mg·kg -1 ·d -1 ) or same dosage vehicle(corn oil) from 4 days preoperation to 10 days postoperation.Animals were sacrificed and the grafted veins were harvested at 7,14 days,respectively after the operation .The grafted veins were then processed for staining and measurements.Hyperplasia,smooth muscle cell proliferation were detected by pathological and immunohistochemical methods.All the data were analyzed by a computerized system.The presence of apoptotic smooth muscle cells was demonstrated by terminal deoxynucleotidyl transferase biotin nick end-labeling (TUNEL) method.Results There was a significant decrease in the average intimal thickness at 7,14 days in the atRA group.Immunohistochemical analysis of proliferating cell nuclear antigen (PCNA) indicated decreased positive cells in the atRA group, compared with the control group at 1 or 2 weeks after the operation. Apoptosis of smooth muscle cells was higher in the atRA group than in the control group at 1 week or 14d postoperation.Conclusions These preliminary results demonstrated that atRA (10mg/kg/d) inhibits smooth muscle cell proliferation and induces smooth muscle cell apoptosis in rats.
出处
《中国医师杂志》
CAS
2003年第9期1158-1159,共2页
Journal of Chinese Physician
关键词
全反式视黄酸
移植静脉
内膜增生
凋亡
狭窄
All-trans-retinoic acid
Grafting vein
Neointimal,proliferation
Apoptosis
Stenosis
Rat
