摘要
目的 探讨c fos、c jun、c myc等早期应答基因与移植静脉平滑肌细胞 (SMC)增殖的影响。方法 建立Wistar大鼠 (6 0只 )自体血管移植模型 ,移植后 2h、6h、2d、1周、2周、4周分别切取移植静脉 ,应用免疫组织化学方法动态观察早期应答基因的变化情况。结果 正常血管平滑肌细胞中鲜有早期应答基因表达 ,c fos、c jun于移植后 2h达高峰 ,吻合口处为 (13.1± 3.3) %、(12 .8±2 .9) % ,中段静脉为 (10 .3± 2 .8) %、(10 .0± 1.9) % ,与术后 1周相比差异有显著性 (P <0 .0 1) ;c myc于 2h有少量表达 ,于 1周时达高峰 [吻合口 (16 .2± 3.8) %、中段静脉 (11.2± 2 .3) % ],与术后 4周相比差异有显著性 (P <0 .0 1)。结论 在移植静脉中 ,早期应答基因是SMC增殖的早期反应基因 ,参与DNA复制 ;c myc可能参与了细胞的持续增殖。
Objective\ To investigate the effect of expression of immediate early gene (c fos, c jun, c myc )products on smooth muscle cell (SMC) proliferation in vein graft. Methods\ Autogenous transforming vein models for 60 Wistar rats was developed. The vein grafts were harvested at 2 h, 6 h, 2 days, 1 week, 2 weeks and 4 weeks after grafting. The change of protein of immediate early gene was detected by using immunohistochemistry. Results\ There was little expression in normal vessels. The protein of c for and c jun peaked at 2 h after the grafting, (13.1±3.3)%, (12.8±2.9)% in anastomasis and (10.3±2.8)%, (10.0±1.9)% in middle of the vein graft showing a significant difference between 2 h and 1 week ( P <0.01). The protein of c myc peaked at 1 week (16.2±3.8)% in anastomasis and (11.2±2.3)% in middle of the vein graft and there was significant difference between 1 week and 4 week ( P <0.01). Conclusion\ In vein graft, immediate early gene is an early responsive gene in SMC entrance from silent to proliferation stage. Immediate early gene perhaps participates in replication of DNA.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第2期151-152,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金!( 3 95 70 691)
辽宁省自然科学基金
