摘要
本文利用流式细胞仪研究了雷公藤多甙(TW)对淋巴细胞膜表面标志及对小鼠脾细胞产生白细胞介素2(IL-2)水平和产生抗休效价的影响,结果证实TW在体外可以抑制小鼠的T淋巴细胞,但不影响B淋巴细胞;Tw还能抑制小鼠脾细胞产生IL-2的能力。在体内,TW明显抑制小鼠的T和B淋巴细胞及抗体效价,并且也同样抑制小鼠脾细胞产生IL-2的水平。
To clarify the mechanism of therapeutic effect of TW on glomerular disease, both in vitro & in vivo investigations were made in mice to observe the sup poressive effects fp this drug on various subpopulations of lymphocytes. Spleen cells from C57BL/6 J mice were used for culture study with TW ( 1 μg/ml) added to the media at different wells. It was found that under the stimulating effect of ConA
( 5 μg/ml) , proliferation of Ly_2 (suppressive T cell) and L_3T_4 (helper T cell) were inhibited by this drug, but 6B_2 population (B cell) was apparently not influenced. In vivo study was also conducted in C57BL/6 J mice. TW in the dosage of 30mg/kg was administered per OS with mice spleen cells obtained on 14th day. Sub-populations of lymphocytes including Thy_(1.2). Ly_2, L_3T_4 and 6B_2 were identified and counted with flow cytometry(FACS440). It was found that the values of all these subpopulations of lymphocytes were significantly lower than the controls.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1992年第3期145-147,共3页
Immunological Journal
关键词
雷公藤多甙
淋巴细胞
白细胞介素
Tryptergium wilfordii hook (TW) , T & B lymphocyte, IL-2
