摘要
本文采用免疫印迹、ELISA和一些生化手段测定慢性浅表性胃炎(CSG)、慢性萎缩性胃炎(CAG)和胃癌(GC)患者的胃液、粪便、血清中CL-3单克隆抗体相关抗原的含量、分子量和阳性率,并对其特性进行部份分析。胃液SDS-PAGE图谱显示CSG组无蛋白区带,CAG组甚少区带,GC组可达十来条区带,甚至融合成片。胃液免疫印迹图谱显示同样结果,其阳性区带分子量均值是:CSG组0,CAG组31KD,GC组40KD;阳性率分别是:0%,44％和72%。胃癌患者粪便、血清中CL-3对应抗原的分子量与胃液的相似,其阳性率是69％和70％。同一患者三种样品中CL-3对应抗原的性质均是涎酸糖蛋白,但其涎酸、糖链的含量不同,且有个体差异。CL-3与CEA和S-Tn抗原均有反应,文中对此展开了讨论。
In the study presented here, SDS-PAGE analysis of gastric juice from patients with various gastric diseases revealed that: no protein band in gastric juice of patients with superficial gastritis (CSG) , few in Chronic atrophic gastritis(CAG), and more than ten bands or even flaky melt in gastric cancer(GC). This was further confirmed by Western-Blot using monoclonal antibody CL-3, which showed the average molecular weight were 3lKD in CAG and 40KD in GC, still no band in CSG. The positive ratio were 0, 44% and 72% in CSG, CAG and GC respectively. The molecular weight of CL-3 related antigen in feces and sera were the same as in gastric juice. The positive ratio were 69% in feces and 70% in sera of gastric cancer. Characterization of CL-3 related antigen in gastric juice, feces and sera from the same patient were coincidence incidence. All of them are Sialyl-glycoprotein, but the concentration of sailic acid and oligosaccharides are not same, which are different among the individuals.CL-3 can not only react with CEA, but also react with S-Tn (Sialylα2-6 GalNAc) antigen. The discussion on these results was presented.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1992年第3期177-181,共5页
Immunological Journal
关键词
单克隆抗体
免疫印迹法
胃肿瘤
Monoclonal antibody, Gastric canecr, CEA & S-Tn antigen, Immunoblotting
