摘要
研究了典型光活化毒素α-三噻吩(简称α-T)对棉铃虫Helicoverpaarmigera和亚洲玉米螟Ostriniafurnacalis谷胱甘肽S-转移酶(GSTs)的影响。结果表明:近紫外光照(300～400nm)对棉铃虫和亚洲玉米螟幼虫GSTs活性无显著影响,但两种昆虫GSTs对α-T的反应不同。无光照条件下,α-T对亚洲玉米螟离体GSTs活性没有影响,而高浓度α-T能抑制棉铃虫离体GSTs活性;高剂量α-T可使两种昆虫活体GSTs活性升高。光照条件下,高浓度和高剂量α-T抑制棉铃虫GSTs活性,而不影响亚洲玉米螟离体GSTs活性,但低剂量α-T抑制其活体活性,而高剂量α-T则诱导其活性增加。棉铃虫和亚洲玉米螟GSTs对α-T反应的差异,可能与它们对药剂的敏感性以及药剂在两者体内的穿透、运转、贮藏、代谢等生理特性上的差异有关。
The effects of αterthienyl (αT) on the glutathione Stransferases(GSTs) were investigated in Helicoverpa armigera and Ostrinia furnacalis. Results indicated that near ultraviolet irradiation (300~400 nm) had no effects on GSTs activities in the control larvae of H. armigera and O.furnacalis both in vitro and in vivo. Treatment with high concentration of αT inhibited the GSTs in vitro in H. armigera, but had no effects in O.furnacalis. Following topical administration, high doses of αT increased the GSTs activities in vivo both in two insects. Upon ultraviolet irradiation, high concentration of αT decreased the GSTs activities in vitro in H. armigera, But had no effects in O.furnacalis. After topical application, high dose of αT reduced the activity in vivo in H. armigera but induced it in O.furnacalis. The differential response of GSTs against αT in H. armigera and in O.furnacalis may be interpreted by their different sensitivities to αT. And the variances in penetration, transportation, storage and metabolism of αT may be also contributed to the differentiation.
出处
《农药学学报》
CAS
CSCD
2003年第3期76-79,共4页
Chinese Journal of Pesticide Science
基金
国家自然科学基金(39900094)
天津市自然科学基金(003701311)资助课题.
关键词
α-三噻吩
棉铃虫
亚洲玉米螟
谷胱甘肽S-转移酶
α-terthienyl
Helicoverpa armigera
Ostrania furnacalis
glutathione S-transferases
