摘要
采用Pichiapastoris(毕赤酵母)表达系统表达拟南芥β-葡萄糖苷酶基因(AT5g44640),获得了有活性的目的蛋白.研究了影响β-葡萄糖苷酶基因表达的因素.结果表明,表达载体整合拷贝数、不同P.pastoris菌株、甲醇体积分数、诱导培养时间和诱导培养起始的D(600nm)等对β-葡萄糖苷酶的产量都有一定的影响.
One βglucosidase gene in Arabidopsis thaliana (AT5g44640) was expressed using Pichia pastoris expression system and the active target protein was obtained. The factors affecting the expression of Pichia were also analyzed. The results showed that copy number of insertion of the expression cassette, strains of P.pastoris, different methanol concentrations, time of induction and beginning concentration of induction all had influence on the yield of βglucosidase.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2003年第3期331-335,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
美国NSF2010年计划项目.
