摘要
用吸光光度法研究了水溶苯胺蓝(AnB)与牛血清白蛋白(BSA)在Britton-Robinson广泛pH缓冲介质中的结合反应,在最佳试验条件下,以试剂空白为参比,AnB-BSA复合物在600nm波长处的吸光度,与BSA的浓度呈良好的线性关系,BSA的线性范围为1.6×10-8～2.4×107mol.L-1,摩尔吸光系数ε=1.34×106L·mol-1·cm-1,Sandell灵敏度为0.051μg·cm-2,BSA的检出限为1.0×10-8mol·L-1,方法具有灵敏度较高、选择性好等特点,用于人血清样品中蛋白质的测定,与溴甲酚绿方法结果一致。
In a solution buffered with B-R buffer solution at pH 2. 1, bovine serum albumin (BSA) reacts with water soluble aniline blue (AnB) to form a complex compound leading to a decrease of absorbance at 600nm, which is the absorption maxima of aniline blue. The absolute values of ΔA at 600nm keep a linear relationship with the concentration of BSA in the range of 1. 6 × 10-8-2. 4 ×10-7 mol ·L-1, and the molar absorptivity is 1. 34× 106L·mol-1 · cm-1. The sandell sensitivity calculated is 0. 051μg ·cm-2. Detection limit of this method (for BSA) is 1. 0×10-8mol · L-1. Similar results (i. e. the linearity, sensitivity and detection limit) are obtained in the determination of human serum albumin (HSA) under the optimum condition. The analytical results obtained by this method were in conformity with those obtained by the bromocresol green method, and showed that the method proposed has high sensitivity and selectivity.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2003年第8期455-457,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
