摘要
目的 研究猫低速弹颅脑伤(LMCW)后脑微循环的改变以及20％甘露醇对脑微循环障碍的影响。方法 改良Carey法制作猫LMCW模型,将12只猫分为单纯创伤组(C组)和甘露醇治疗组(Man组)。微循环显微镜活体实时观察软脑膜微循环并测量软脑膜细动脉(Da)、细静脉(Dv)管径;测量微区CBF;监测生命体征;采血测算重要血液流变学指标,8h后观察创伤对侧组织病理学变化。结果 猫低速弹颅脑伤后Da、Dv收缩持续30min,之后C组微血管扩张至2h时恢复正常,5～8hDa痉挛,Dv扩张、瘀血;各血液流变学指标均明显异常,至8h仍未恢复。结论 20％甘露醇通过改善血液的流变学特性,减轻了颅脑伤后的脑微循环障碍和病理损害。
Objective To seek the alterations of cerebral microcirculation following low missile craniocerebral wound (LMCW) in cats and observe the influence of mannitol on cerebral microcirculation. Methods A cat model was constructed in reference to Carey MEs method. 12 cats were evenly divided into two groups: LMCW group and mannitol group. Pial microcirculation, pial microvessel caliber, microregion cerebral blood flow (CBF) and vital signs were monitored in vivo. Blood samples were collected for surveying and calculating important rheological parameters. All animals were executed by air injection 8 h after LMCW. Brain tissues and brain microvascular corrosion casts were preserved for light, electronic microscope observation. Results Pial aterioles and venules constricted immediately and prolonged for 30 minuts. In LMCW group, they dilated later and resumed the normal level at 2 hours, with arteriole spasm and venule dilation occuring from 5 hours to 8 hours. All blood rheological parameters were abnormal but didn't recover to normal 8 hours later. Conclusion Mannitol can alleviate the impaired cerebral microcirculation and pathological damage caused by LMCW through improving its blood rheological characters.
出处
《中华神经医学杂志》
CAS
CSCD
2003年第4期282-284,277,共4页
Chinese Journal of Neuromedicine
基金
军队九五重点课题(01Z054)
关键词
颅脑伤
脑微循环
甘露醇
实验研究
血液流变学
craniocerebral wound
low velocity missile
brain
microcirculation
mannitol
