摘要
目的 :探讨从血小板源生长因子 -B链 (PDGF -B)的基因水平阻断对肺血管平滑肌细胞增殖的影响。方法 :给肺动脉平滑肌细胞 (VSMC)分别施加不同剂量的c -sis癌基因反义寡核苷酸 (ASON) ,通过四甲基偶氮唑蓝 (MTT)法观察细胞不同时相的增殖曲线 ;采用流式细胞术观察不同干预条件下VSMC细胞周期的变化。结果 :中、大浓度ASON对细胞增殖有明显抑制作用。在中浓度的ASON作用下 ,加入PDGF -BB能促进VSMC的增殖。ASON干预前后 ,VSMC增殖周期中各期细胞构成发生显著的变化 ,以G0 +G1期细胞增多、S +G2 +M期细胞减少为特征。各组的G0 +G1期细胞均显著多于对照组 (P <0 0 5 )。小剂量与中剂量、中剂量与大剂量间G0 +G1期细胞有显著差异 (P <0 0 5 )。结论 :中、大剂量ASON能明显抑制肺VSMC的增殖 ,可使G0 +G1期细胞数明显增多 。
AIM: To investigate the effect of antisense oligonucleotides(ASON) of c-sis on cellular cycle and proliferation of pulmonary artery vascular smooth muscle cells(VSMC).METHODS: Tissue mass culture was done to get VSMC of pulmonary artery. Different concentrations of antisense oligonucleotides of c-sis were added into the cultures to observe the VSMC proliferation curve using MTT test. The changes of VSMC cellular cycle were also observed by flow cytometry. RESULTS: ASON with mid-to high concentrations restrained the proliferation of VSMC apparently with the peak of cell growth being attenuated or eliminated. Affected by mid-concentration ASON, PDGF-BB showed significant accelerating effect on the proliferation of VSMC. The ratio of G 0/G 1 in cellular cycle was increased significantly in VSMC culture with ASON in comparison with control. The G 0/G 1 ratio also showed significant differences among different concentration of ASON groups( P<0.05 ). CONCLUSION: Mid-to high concentration of ASON was a powerful inhibitor of cellular proliferation for pulmonary artery VSMC. ASON increased the ratio of G 0/G 1 significantly and the increase seems to be ASON dosage dependent.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2003年第9期1227-1230,共4页
Chinese Journal of Pathophysiology
基金
四川大学校青年基金资助 (No.4 32 0 0 3)
