摘要
目的 :探讨骨巨细胞瘤 (GCT)石蜡切片中端粒酶活性检测的可行性以及端粒酶在GCT生物学特性中的意义。方法 :采用针对人端粒酶hTERT寡核苷酸探针序列 ,对 2 7例GCT石蜡标本进行原位杂交 ,检测端粒酶逆转录酶 (hTERT)mRNA的表达情况。结果 :2 7例GCT中 8例hTERTmRNA表达阳性 ,石蜡切片中基质细胞、部分多核巨细胞hTERTmRNA表达阳性。结论 :采用原位杂交技术检测GCT石蜡切片中hTERT表达 。
AIM: human telomerase reverse transcriptase (hTERT) is the catalytic subunit of telomerase, and hTERT mRNA expression contributes to telomerase activity. We therefore assessed the significance of hTERT expression in the biological behavior of giant cell tumor(GCT) of bone. METHODS: In situ hybridization was used to identify the expression of hTERT mRNA in 27 formalin-fixed paraffin-embedded clinical specimens from human GCT. RESULTS: 8 cases (8/27)displayed positive hTERT mRNA signal, positive hTERT mRNA signal located in the cytoplasms of mononuclear stromal cells and some multinuclear giant cells. CONCLUSION: The expression of telomerase in paraffin-embedded tissue can be evaluated efficiently and effectively by in situ hybridization, and the results suggest that there may be a neoplastic multinuclear giant cell type in human GCT.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2003年第9期1224-1226,T004,共4页
Chinese Journal of Pathophysiology
基金
广东省卫生厅资助项目 (C2 0 0 1182 )
关键词
巨细胞瘤
骨
端粒
末端转移酶
原位杂交
Giant cell tumor of bone
Telomerase
In situ hybridzation
