摘要
目的:研究骨肿瘤中端粒酶相关基因中,人端粒酶催化亚单位(humantelomerasecatalyticsubunit,hTERT)和人端粒酶相关蛋白(humantelomeraseassociatedprotein,TP1)两个重要成分的表达及对评估肿瘤良、恶性的意义。方法:提取50例手术切除骨肿瘤组织、2种人成骨肉瘤细胞系Saos-2、OS732和原代培养人成骨细胞的总RNA。用一步RT-PCR方法检测hTERT和TP1mRNA的表达。结果:所有骨肿瘤标本及细胞系均检测到TP1的表达。hTERT在17例良性骨肿瘤、33例骨肉瘤、2种人成骨肉瘤细胞系和人成骨细胞的表达率分别为64.7%、57.6%、0%和0%。良、恶性骨肿瘤间hTERT表达的差异不具有显著性(P>0.05),不同分化程度骨肉瘤间hTERT表达的差异也不具有显著性(P>0.05)。结论:一步RT-PCR是检测端粒酶亚单位的有效方法。hTERT和TP1表达水平的上调在骨肿瘤恶性进展中可能不起主要作用,在骨肿瘤发展和维持中可能有替代性(ALT)机制导致细胞永生化。
Objective: To investigate the expression level of two major components of the telomerase associated genes: human telomerase catalytic subunit (hTERT) and human telomerase associated protein (TP1) in bone tumoss and its significance in evaluating tumor malignancy. Methods: Total RNA was obtained from 50 surgical bone tumors, 2 human osteoblastic osteosarcoma cell lines (Saos 2 and OS732) and a primary human osteoblast culture. The hTERT and TP1 mRNA were analyzed using method of one step reverse transcriptase polymerase chain reaction (RT PCR) . Results: The TP1 expression was found in all samples examined. In contrast, hTERT expression was detected in 11 of 17 benign bone tumors (64.7%) and in 19 of 33 skeletal sarcomas (57.6%). No hTERT was expressed in 2 human osteosarcoma cell lines, nor in the human primary osteoblast (0%). There was no remarkable difference in hTERT expression between benign and malignant bone tumors (P >0.05). Meanwhile, no notable difference in hTERT expression had been observed among different differentiated types of skeletal sarcomas (P >0.05). Conclusions: One step RT PCR is a powerful method for detecting telomerase subunits. The up regulation of hTERT and TP1 expression may not play a critical role in bone tumor malignant progression. There might be an alternative mechanism for cell immortalization, yet to be determined, in the development and maintenance of bone tumors.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2001年第9期943-947,共5页
Chinese Journal of Cancer
