摘要
目的 :对影响人参 (Panaxginseng)毛状根生长及人参皂苷合成的培养条件进行了研究。 方法 :比较了不同培养基上人参毛状根和人参皂苷的含量变化。结果 :人参毛状根在MS +IBA0 .5mg/l的培养液里预培养 72h后 ,再转入MS基本培养液培养 ,经过 6周的培养 ,人参总皂苷含量达到 5 .1 90 % ,人参皂苷 (Re+Rg)含量达到了 0 .32 71 % ,人参毛状根干重增加 98.74倍。结论 :确定了最佳的培养基和培养条件 。
Objective: To study the effects of physical and chemical factors on hairy root of Panax ginseng and its glycoside production. Methods: The cell growth index and its glycoside yield in different culture condition such as different plant hormones mixed, mediums and time were compared. Results: After hairy root of Panax ginseng was precultivated on the MS medium with IBA (0.5%) at first 72 hours. the culture was cultured on the MS medium. During 6 weeks, dry weight of hairy root increased by 98.74 times, and the content of glycoside synthesized by hairy root is 5.190% and Re+Rg1 is 0.3271%, respectively. Conclusion:We have achieved the best condition of hairy root of Panax ginseng.
出处
《中成药》
CAS
CSCD
北大核心
2003年第9期746-748,共3页
Chinese Traditional Patent Medicine
关键词
人参
发根农杆菌
毛状根
人参皂苷
ginseng
agrobacterium rhizogenes
hairy root
glycoside
