摘要
用 4 0 8nm的LED诱导不同浓度人全血溶液的荧光光谱 ,包括研究了血液的自吸收对其荧光光谱结构产生的影响 ;分析了血液的荧光光谱随其浓度增加出现红移的现象并进行了解释 ;提出了 5 5 6nm谱峰不是某一荧光团所产生的特征峰 ,而是由吸收所造成的假峰的观点 ;讨论了激励光散射中心波长偏离激励光中心波长现象的原因。
The fluorescent spectra of human blood solution with different concentrations induced by 408nmLED are reported in this paper, including the investigation of the influence on fluorescent profile exerted by blood self-absorption. The phenomena that fluorescence spectral peaks are apparently red-shifted with the increment of blood concentration are analyzed and the mechanism is given a reasonable explanation. The viewpoint is put forward that 556nm spectrum is illusive owing to blood absorption but not a characteristic fluorescence emitted by a certain fluorophore. The reasons that the central wavelength of dispersion light departures the one of exciting-light are discussed. The research result may have reference value to light induced tissue autofluorescence diagnosis techniques.
出处
《应用激光》
CSCD
北大核心
2003年第4期232-234,242,共4页
Applied Laser
