摘要
在微生物资源的开发研究过程中 ,利用TLC方法 ,配合TLC数据库的检索 ,对嗜碱放线菌菌株YIMGQ1 4的次生代谢产物进行了研究。并从中分离纯化出两个具有生物活性的专利化合物“UmycinC”和“UmycinB”。结果证明 :相对于常规的生物活性筛选 ,TLC法在微生物次生代谢产物研究中具有快速、简便、经济的特点 ,适合于小量常规筛选的目的。通过进一步对相关的TLC数据库进行构建和补充 ,将能极大地改善该方法的使用效率。
In the course of the microbial resources exploitation, the secondary metabolites research of the alkalophilic strain YIMGQ-14 was carried on using the TLC method and with the coordinate of the TLC database searching. Two patented biological active compounds “Umycin C” and “Umycin B” were isolated and purified. The result showed that, compared with the ordinary biological screening, the TLC screening is fast, convenient, economical and much more suitable for the small scale daily screening purpose. With the reconstruction and supplement of the related TLC data, the working efficiency of this method can be improved greartly.
出处
《微生物学报》
CAS
CSCD
北大核心
2003年第4期481-486,共6页
Acta Microbiologica Sinica
基金
国家科技部重点项目 ( 2 0 0 1CCC0 0 60 0 )
云南省自然科学基金 ( 2 0 0 1C0 0 3R)~~
