摘要
近几年,叶绿体DNA的研究越来越受到重视,因为叶绿体不仅是植物光合作用的器官,而且DNA分子结构紧凑,相对于核DNA而言,分子量小,结构简单,更易从分予水平上作精细分析。另外,叶绿体DNA具有稳定性、进化缓慢性及编码序列的保守性,因而是研究植物系统发育的好材料。虽然在许多植物中,叶绿体DNA分子非常保守。
Chloroplast DNA (cpDNA) were prepared from five Poplar samples (two hybrids of Populus deltoides Bartr. CL. 'Lux' x P. deltoides Bartr. CL. 'Harvard', two natural hybrids of P. deltoides and one sample of P. laurifolia) and analysed with five restriction enzymes. The results show that although the cpDNA diversity between Sect. Tacamahaca Spach. and Sect. Aigeiros Duby is low, they can be distinguished by using the restriction fragment length polymorphisms (RFLP) generated by EcoR I and BamH I . The RFLP generated by BamH I can also be used to distinguish the two natural hybrids of P. deltodes. So the analysis of Poplar cpDNA RFLP provides a molecular biological method for Poplar classification, cross breeding program and phylogenetic studies.
出处
《林业科学研究》
CSCD
北大核心
1992年第4期465-468,共4页
Forest Research
关键词
杨树
叶绿体
DNA
提取
RFLP
Poplar chloroplast DNA restriction fragment length polymorphisms (RFLP)
